Title |
Human serum inhibits adhesion and biofilm formation in Candida albicans
|
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Published in |
BMC Microbiology, March 2014
|
DOI | 10.1186/1471-2180-14-80 |
Pubmed ID | |
Authors |
Xiurong Ding, Zhizhong Liu, Jianrong Su, Donghui Yan |
Abstract |
Candida albicans can form biofilms on intravenous catheters; this process plays a key role in the pathogenesis of catheter infections. This study evaluated the effect of human serum (HS) on C. albicans biofilm formation and the expression of adhesion-related genes in vitro. A C. albicans laboratory strain (ATCC90028) and three clinical strains were grown for 24 h in RPMI 1640 supplemented with HS or RPMI 1640 alone (as a control). The growth of biofilm cells of four strains was monitored by a Live Cell Movie Analyzer, and by XTT reduction assay. The expression of the adhesion-related genes BCR1, ALS1, ALS3, HWP1 and ECE1 was analyzed by RT-PCR at three time points (60 min, 90 min, and 24 h). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Venezuela, Bolivarian Republic of | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Poland | 2 | 2% |
Russia | 1 | 1% |
India | 1 | 1% |
Unknown | 85 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 24 | 27% |
Student > Master | 13 | 15% |
Researcher | 12 | 13% |
Student > Bachelor | 11 | 12% |
Student > Doctoral Student | 4 | 4% |
Other | 10 | 11% |
Unknown | 15 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 27% |
Biochemistry, Genetics and Molecular Biology | 11 | 12% |
Immunology and Microbiology | 9 | 10% |
Medicine and Dentistry | 8 | 9% |
Veterinary Science and Veterinary Medicine | 3 | 3% |
Other | 12 | 13% |
Unknown | 22 | 25% |