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Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA

Overview of attention for article published in BMC Molecular Biology, July 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#9 of 310)
  • High Attention Score compared to outputs of the same age (82nd percentile)

Mentioned by

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8 tweeters
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1 patent
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2 Wikipedia pages

Citations

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15 Dimensions

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27 Mendeley
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Title
Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA
Published in
BMC Molecular Biology, July 2017
DOI 10.1186/s12867-017-0096-x
Pubmed ID
Authors

Leslie C. Sutherland, Philippe Thibault, Mathieu Durand, Elvy Lapointe, Jose M. Knee, Ariane Beauvais, Irina Kalatskaya, Sarah C. Hunt, Julie J. Loiselle, Justin G. Roy, Sarah J. Tessier, Gustavo Ybazeta, Lincoln Stein, Rashmi Kothary, Roscoe Klinck, Benoit Chabot

Abstract

RBM10 is an RNA binding protein involved in message stabilization and alternative splicing regulation. The objective of the research described herein was to identify novel targets of RBM10-regulated splicing. To accomplish this, we downregulated RBM10 in human cell lines, using small interfering RNAs, then monitored alternative splicing, using a reverse transcription-PCR screening platform. RBM10 knockdown (KD) provoked alterations in splicing events in 10-20% of the pre-mRNAs, most of which had not been previously identified as RBM10 targets. Hierarchical clustering of the genes affected by RBM10 KD revealed good conservation of alternative exon inclusion or exclusion across cell lines. Pathway annotation showed RAS signaling to be most affected by RBM10 KD. Of particular interest was the finding that splicing of SMN pre-mRNA, encoding the survival of motor neuron (SMN) protein, was influenced by RBM10 KD. Inhibition of RBM10 resulted in preferential expression of the full-length, exon 7 retaining, SMN transcript in four cancer cell lines and one normal skin fibroblast cell line. SMN protein is expressed from two genes, SMN1 and SMN2, but the SMN1 gene is homozygously disrupted in people with spinal muscular atrophy; as a consequence, all of the SMN that is expressed in people with this disease is from the SMN2 gene. Expression analyses using primary fibroblasts from control, carrier and spinal muscle atrophy donors demonstrated that RBM10 KD resulted in preferential expression of the full-length, exon 7 retaining, SMN2 transcript. At the protein level, upregulation of the full-length SMN2 was also observed. Re-expression of RBM10, in a stable RBM10 KD cancer cell line, correlated with a reversion of the KD effect, demonstrating specificity. Our work has not only expanded the number of pre-mRNA targets for RBM10, but identified RBM10 as a novel regulator of SMN2 alternative inclusion.

Twitter Demographics

The data shown below were collected from the profiles of 8 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 33%
Student > Ph. D. Student 5 19%
Student > Doctoral Student 3 11%
Student > Master 3 11%
Professor 1 4%
Other 2 7%
Unknown 4 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 33%
Agricultural and Biological Sciences 5 19%
Nursing and Health Professions 3 11%
Neuroscience 2 7%
Social Sciences 1 4%
Other 1 4%
Unknown 6 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2022.
All research outputs
#2,528,509
of 21,035,197 outputs
Outputs from BMC Molecular Biology
#9
of 310 outputs
Outputs of similar age
#49,067
of 285,683 outputs
Outputs of similar age from BMC Molecular Biology
#1
of 1 outputs
Altmetric has tracked 21,035,197 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 310 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 285,683 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them