Title |
Simultaneous multi-analyte urinary protein assay for bladder cancer detection
|
---|---|
Published in |
BMC Biotechnology, April 2014
|
DOI | 10.1186/1472-6750-14-24 |
Pubmed ID | |
Authors |
Charles J Rosser, Yunfeng Dai, Makito Miyake, Ge Zhang, Steve Goodison |
Abstract |
The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of a myriad of clinical assays. Here, we tested the performance of a new, multiplex protein array platform to quantitate three bladder cancer-associated proteins in urine samples. The following analytes, interleukin 8 (IL8), matrix metallopeptidase 9 (MMP9), and vascular endothelial growth factor A (VEGFA) were monitored using Q-plex, a customized multiplex ELISA system from Quansys Biosciences, and individual target commercial ELISA kits. The performance of the two approaches was compared by evaluating the diagnostic accuracy of the biomarker assays in samples from a cohort of 73 subjects of known bladder cancer status. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 28% |
Student > Ph. D. Student | 4 | 16% |
Student > Master | 3 | 12% |
Lecturer | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Other | 3 | 12% |
Unknown | 4 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 5 | 20% |
Biochemistry, Genetics and Molecular Biology | 5 | 20% |
Nursing and Health Professions | 2 | 8% |
Agricultural and Biological Sciences | 2 | 8% |
Chemistry | 2 | 8% |
Other | 4 | 16% |
Unknown | 5 | 20% |