Title |
MiR-661 promotes tumor invasion and metastasis by directly inhibiting RB1 in non small cell lung cancer
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Published in |
Molecular Cancer, July 2017
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DOI | 10.1186/s12943-017-0698-4 |
Pubmed ID | |
Authors |
Feiye Liu, Yanjun Cai, Xiaoxiang Rong, Jinzhang Chen, Dayong Zheng, Lu Chen, Junyi Zhang, Rongcheng Luo, Peng Zhao, Jian Ruan |
Abstract |
Aberrant microRNA expression has been implicated in metastasis of cancers. MiR-661 accelerates proliferation and invasion of breast cancer and ovarian cancer, while impedes that of glioma. Its role in non small cell lung cancer (NSCLC) and underlying mechanism are worthy elucidation. Expression of miR-661 was measured with real-time PCR in both NSCLC tissues and cell lines. The effects of miR-661 on migration, invasion and metastasis capacity of NSCLC were evaluated using wound healing, transwell assay and animal models. Dual reporter luciferase assay and complementary experiments were performed to validate RB1 as a direct target of miR-661 for participation in the progression of NSCLC. MiR-661 was upregulated in NSCLC tissues as compared to paired adjacent tissues and associated with shorter overall survival. Furthermore, miR-661 promoted proliferation, migration and metastasis of NSCLC. Then, we identified RB1 as a direct target of miR-661 through which miR-661 affected EMT process and metastasis of NSCLC. RB1 interacted with E2F1 and both could mediate EMT process in NSCLC. MiR-661 promotes metastasis of NSCLC through RB/E2F1 signaling and EMT events, thus may serves as a negative prognostic factor and possible target for treatment of NSCLC patient. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 21% |
Student > Master | 5 | 17% |
Student > Bachelor | 2 | 7% |
Researcher | 2 | 7% |
Student > Doctoral Student | 2 | 7% |
Other | 4 | 14% |
Unknown | 8 | 28% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 28% |
Medicine and Dentistry | 6 | 21% |
Agricultural and Biological Sciences | 3 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
Business, Management and Accounting | 1 | 3% |
Other | 2 | 7% |
Unknown | 7 | 24% |