Title |
Comparison of next generation sequencing technologies for transcriptome characterization
|
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Published in |
BMC Genomics, August 2009
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DOI | 10.1186/1471-2164-10-347 |
Pubmed ID | |
Authors |
P Kerr Wall, Jim Leebens-Mack, André S Chanderbali, Abdelali Barakat, Erik Wolcott, Haiying Liang, Lena Landherr, Lynn P Tomsho, Yi Hu, John E Carlson, Hong Ma, Stephan C Schuster, Douglas E Soltis, Pamela S Soltis, Naomi Altman, Claude W dePamphilis |
Abstract |
We have developed a simulation approach to help determine the optimal mixture of sequencing methods for most complete and cost effective transcriptome sequencing. We compared simulation results for traditional capillary sequencing with "Next Generation" (NG) ultra high-throughput technologies. The simulation model was parameterized using mappings of 130,000 cDNA sequence reads to the Arabidopsis genome (NCBI Accession SRA008180.19). We also generated 454-GS20 sequences and de novo assemblies for the basal eudicot California poppy (Eschscholzia californica) and the magnoliid avocado (Persea americana) using a variety of methods for cDNA synthesis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 25 | 4% |
United Kingdom | 16 | 2% |
Brazil | 12 | 2% |
France | 7 | <1% |
Germany | 6 | <1% |
Spain | 4 | <1% |
Japan | 4 | <1% |
Netherlands | 3 | <1% |
Canada | 3 | <1% |
Other | 28 | 4% |
Unknown | 597 | 85% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 228 | 32% |
Student > Ph. D. Student | 171 | 24% |
Professor > Associate Professor | 64 | 9% |
Student > Master | 60 | 9% |
Professor | 40 | 6% |
Other | 100 | 14% |
Unknown | 42 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 497 | 70% |
Biochemistry, Genetics and Molecular Biology | 61 | 9% |
Medicine and Dentistry | 22 | 3% |
Computer Science | 18 | 3% |
Engineering | 14 | 2% |
Other | 40 | 6% |
Unknown | 53 | 8% |