Title |
A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper
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Published in |
BMC Ecology and Evolution, September 2003
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DOI | 10.1186/1472-6785-3-7 |
Pubmed ID | |
Authors |
Geoff Hide, Jacqueline M Hughes, Robert McNuff |
Abstract |
The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum was used to investigate a rapid and simple detection system based on polymerase chain reaction amplification (PCR) from organisms immobilised on FTA paper. Using primers designed from the alpha-tubulin genes of Blepharisma, specific and sensitive detection to the equivalent of a single Blepharisma cell could be achieved. Similar detection levels were found using water samples, containing Blepharisma, which were dried onto Whatman FTA paper. This system has potential as a sensitive convenient detection system for Blepharisma and could be applied to other protozoan organisms. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 6% |
Unknown | 15 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 56% |
Researcher | 2 | 13% |
Student > Master | 2 | 13% |
Student > Postgraduate | 1 | 6% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 44% |
Environmental Science | 2 | 13% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Energy | 1 | 6% |
Other | 2 | 13% |
Unknown | 2 | 13% |