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Purification of infectious human herpesvirus 6A virions and association of host cell proteins

Overview of attention for article published in Virology Journal, October 2007
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Purification of infectious human herpesvirus 6A virions and association of host cell proteins
Published in
Virology Journal, October 2007
DOI 10.1186/1743-422x-4-101
Pubmed ID

Maria Hammarstedt, Jenny Ahlqvist, Steven Jacobson, Henrik Garoff, Anna Fogdell-Hahn


Viruses that are incorporating host cell proteins might trigger autoimmune diseases. It is therefore of interest to identify possible host proteins associated with viruses, especially for enveloped viruses that have been suggested to play a role in autoimmune diseases, like human herpesvirus 6A (HHV-6A) in multiple sclerosis (MS). We have established a method for rapid and morphology preserving purification of HHV-6A virions, which in combination with parallel analyses with background control material released from mock-infected cells facilitates qualitative and quantitative investigations of the protein content of HHV-6A virions. In our iodixanol gradient purified preparation, we detected high levels of viral DNA by real-time PCR and viral proteins by metabolic labelling, silver staining and western blots. In contrast, the background level of cellular contamination was low in the purified samples as demonstrated by the silver staining and metabolic labelling analyses. Western blot analyses showed that the cellular complement protein CD46, the receptor for HHV-6A, is associated with the purified and infectious virions. Also, the cellular proteins clathrin, ezrin and Tsg101 are associated with intact HHV-6A virions. Cellular proteins are associated with HHV-6A virions. The relevance of the association in disease and especially in autoimmunity will be further investigated.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 40%
Student > Ph. D. Student 5 17%
Student > Doctoral Student 4 13%
Student > Master 3 10%
Student > Bachelor 2 7%
Other 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 47%
Immunology and Microbiology 6 20%
Medicine and Dentistry 5 17%
Biochemistry, Genetics and Molecular Biology 3 10%
Neuroscience 2 7%
Other 0 0%