Title |
Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes
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Published in |
Biotechnology for Biofuels and Bioproducts, February 2014
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DOI | 10.1186/1754-6834-7-20 |
Pubmed ID | |
Authors |
J Philipp Benz, Ryan J Protzko, Jonas MS Andrich, Stefan Bauer, John E Dueber, Chris R Somerville |
Abstract |
Pectin-rich agricultural wastes potentially represent favorable feedstocks for the sustainable production of alternative energy and bio-products. Their efficient utilization requires the conversion of all major constituent sugars. The current inability of the popular fermentation host Saccharomyces cerevisiae to metabolize the major pectic monosaccharide D-galacturonic acid (D-GalA) significantly hampers these efforts. While it has been reasoned that the optimization of cellular D-GalA uptake will be critical for the engineering of D-GalA utilization in yeast, no dedicated eukaryotic transport protein has been biochemically described. Here we report for the first time such a eukaryotic D-GalA transporter and characterize its functionality in S. cerevisiae. |
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Demographic breakdown
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Mendeley readers
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Unknown | 14 | 11% |