Title |
Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells
|
---|---|
Published in |
Cellular & Molecular Biology Letters, October 2007
|
DOI | 10.2478/s11658-007-0034-x |
Pubmed ID | |
Authors |
Takeo Yamaguchi, Kenji Hashiguchi, Satoshi Katsuki, Wakako Iwamoto, Shoichiro Tsuruhara, Shigeyuki Terada |
Abstract |
We previously demonstrated that caspase-3, an executioner of apoptosis, is activated in the pressure-induced apoptosis of murine erythroleukemia (MEL) cells (at 100 MPa). Here, we examined the pathway of caspase-3 activation using peptide substrates and caspase inhibitors. Using the substrates of caspases-8 and -9, it was found that both are activated in cells under high pressure. The production of nuclei with sub-G1 DNA content in 100 MPa-treated MEL cells was suppressed by inhibitors of caspases-8 and -9, and pan-caspase. In 100 MPa-treated cells, pan-caspase inhibitor partially prevented the cytochrome c release from the mitochondria and the breakdown of mitochondrial membrane potential. These results suggest that the intrinsic and extrinsic pathways are activated in apoptotic signaling during the high pressure-induced death of MEL cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 63% |
Researcher | 1 | 13% |
Lecturer | 1 | 13% |
Student > Postgraduate | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 50% |
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Physics and Astronomy | 1 | 13% |
Medicine and Dentistry | 1 | 13% |