Title |
Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
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Published in |
Acta Veterinaria Scandinavica, March 2014
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DOI | 10.1186/1751-0147-56-18 |
Pubmed ID | |
Authors |
Min Jung Kim, Hyun Ju Oh, Geon A Kim, Young Kwang Jo, Jin Choi, Hye Jin Kim, Hee Yeon Choi, Hyun Wook Kim, Min Cheol Choi, Byeong Chun Lee |
Abstract |
The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 67% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 6 | 17% |
Student > Master | 6 | 17% |
Researcher | 5 | 14% |
Student > Ph. D. Student | 3 | 9% |
Other | 3 | 9% |
Other | 3 | 9% |
Unknown | 9 | 26% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 8 | 23% |
Agricultural and Biological Sciences | 5 | 14% |
Veterinary Science and Veterinary Medicine | 3 | 9% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Engineering | 2 | 6% |
Other | 6 | 17% |
Unknown | 9 | 26% |