Title |
Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat
|
---|---|
Published in |
BMC Genomics, April 2014
|
DOI | 10.1186/1471-2164-15-276 |
Pubmed ID | |
Authors |
Lindsey J Leach, Eric J Belfield, Caifu Jiang, Carly Brown, Aziz Mithani, Nicholas P Harberd |
Abstract |
Bread wheat (Triticum aestivum) has a large, complex and hexaploid genome consisting of A, B and D homoeologous chromosome sets. Therefore each wheat gene potentially exists as a trio of A, B and D homoeoloci, each of which may contribute differentially to wheat phenotypes. We describe a novel approach combining wheat cytogenetic resources (chromosome substitution 'nullisomic-tetrasomic' lines) with next generation deep sequencing of gene transcripts (RNA-Seq), to directly and accurately identify homoeologue-specific single nucleotide variants and quantify the relative contribution of individual homoeoloci to gene expression. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 2% |
United States | 1 | <1% |
Netherlands | 1 | <1% |
Australia | 1 | <1% |
Unknown | 112 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 28% |
Researcher | 32 | 27% |
Student > Master | 8 | 7% |
Student > Doctoral Student | 7 | 6% |
Student > Bachelor | 7 | 6% |
Other | 18 | 15% |
Unknown | 12 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 79 | 68% |
Biochemistry, Genetics and Molecular Biology | 17 | 15% |
Unspecified | 1 | <1% |
Economics, Econometrics and Finance | 1 | <1% |
Social Sciences | 1 | <1% |
Other | 2 | 2% |
Unknown | 16 | 14% |