Title |
A Simplified Up-Down Method (SUDO) for Measuring Mechanical Nociception in Rodents Using von Frey Filaments
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Published in |
Molecular Pain, January 2014
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DOI | 10.1186/1744-8069-10-26 |
Pubmed ID | |
Authors |
Robert P Bonin, Cyril Bories, Yves De Koninck |
Abstract |
The measurement of mechanosensitivity is a key method for the study of pain in animal models. This is often accomplished with the use of von Frey filaments in an up-down testing paradigm. The up-down method described by Chaplan et al. (J Neurosci Methods 53:55-63, 1994) for mechanosensitivity testing in rodents remains one of the most widely used methods for measuring pain in animals. However, this method results in animals receiving a varying number of stimuli, which may lead to animals in different groups receiving different testing experiences that influences their later responses. To standardize the measurement of mechanosensitivity we developed a simplified up-down method (SUDO) for estimating paw withdrawal threshold (PWT) with von Frey filaments that uses a constant number of five stimuli per test. We further refined the PWT calculation to allow the estimation of PWT directly from the behavioral response to the fifth stimulus, omitting the need for look-up tables. |
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Country | Count | As % |
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Japan | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 2 | <1% |
Italy | 1 | <1% |
Portugal | 1 | <1% |
Spain | 1 | <1% |
United Kingdom | 1 | <1% |
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Researcher | 48 | 14% |
Student > Master | 43 | 13% |
Student > Bachelor | 39 | 12% |
Student > Doctoral Student | 20 | 6% |
Other | 47 | 14% |
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Pharmacology, Toxicology and Pharmaceutical Science | 12 | 4% |
Other | 22 | 7% |
Unknown | 85 | 25% |