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Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers

Overview of attention for article published in Virology Journal, June 2011
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Title
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
Published in
Virology Journal, June 2011
DOI 10.1186/1743-422x-8-308
Pubmed ID
Authors

Guillermo A Maroniche, Mónica Sagadín, Vanesa C Mongelli, Graciela A Truol, Mariana del Vas

Abstract

Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers might unveil the molecular basis of viral transmission. Quantitative real-time RT-PCR (RT-qPCR) is currently the most accurate and sensitive method used for quantitative gene expression analysis. In order to normalize the resulting quantitative data, reference genes with constant expression during the experimental procedures are needed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 1%
Unknown 72 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 27%
Researcher 12 16%
Student > Doctoral Student 8 11%
Student > Master 8 11%
Student > Bachelor 5 7%
Other 12 16%
Unknown 8 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 47 64%
Biochemistry, Genetics and Molecular Biology 6 8%
Environmental Science 4 5%
Earth and Planetary Sciences 1 1%
Social Sciences 1 1%
Other 2 3%
Unknown 12 16%