Title |
Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris
|
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Published in |
BMC Research Notes, July 2017
|
DOI | 10.1186/s13104-017-2692-8 |
Pubmed ID | |
Authors |
Stephanie P. Cartwright, Roslyn M. Bill, Bui Tien Sy, Hieu Tran-Van, Hung Minh Nguyen |
Abstract |
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni(2+) affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study. |
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