Title |
Comparison of methods for fecal microbiome biospecimen collection
|
---|---|
Published in |
BMC Microbiology, April 2014
|
DOI | 10.1186/1471-2180-14-103 |
Pubmed ID | |
Authors |
Christine Dominianni, Jing Wu, Richard B Hayes, Jiyoung Ahn |
Abstract |
Effective means are needed to efficiently collect fecal samples for microbiome analysis in large-scale epidemiological studies. Using twenty-four fecal aliquots prepared from three healthy individuals, we compared the following four fecal sample collection methods for assessment of human gut microbiome: 1) fecal occult blood test cards, held at room temperature for three days, 2) Eppendorf tubes, at room temperature for three days, 3) Eppendorf tubes with RNAlater, at room temperature, and 4) as controls, samples immediately frozen at -80°C. The 24 samples were assayed by 16S rRNA gene sequencing to compare overall microbiome structure and taxon distributions according to collection method. |
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Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 7% |
Unknown | 13 | 93% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 12 | 86% |
Practitioners (doctors, other healthcare professionals) | 1 | 7% |
Scientists | 1 | 7% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 2 | <1% |
United States | 2 | <1% |
Norway | 1 | <1% |
South Africa | 1 | <1% |
United Kingdom | 1 | <1% |
Canada | 1 | <1% |
Italy | 1 | <1% |
Taiwan | 1 | <1% |
New Zealand | 1 | <1% |
Other | 2 | <1% |
Unknown | 279 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 69 | 24% |
Student > Ph. D. Student | 58 | 20% |
Student > Master | 37 | 13% |
Student > Bachelor | 26 | 9% |
Other | 19 | 7% |
Other | 50 | 17% |
Unknown | 33 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 95 | 33% |
Biochemistry, Genetics and Molecular Biology | 49 | 17% |
Medicine and Dentistry | 40 | 14% |
Immunology and Microbiology | 23 | 8% |
Environmental Science | 7 | 2% |
Other | 29 | 10% |
Unknown | 49 | 17% |