Title |
Production of recombinant human annexin V by fed-batch cultivation
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Published in |
BMC Biotechnology, April 2014
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DOI | 10.1186/1472-6750-14-33 |
Pubmed ID | |
Authors |
Laura S Marder, Juleane Lunardi, Gaby Renard, Diana C Rostirolla, Guilherme O Petersen, José E S Nunes, Ana Paula D de Souza, Ana Christina de O Dias, Jocelei M Chies, Luiz A Basso, Diógenes S Santos, Cristiano V Bizarro |
Abstract |
Annexin V, a 35.8 kDa intracellular protein, is a Ca⁺²-dependent phospholipid binding protein with high affinity to phosphatidylserine (PS), which is a well-known hallmark of apoptosis. Annexin V is a sensitive probe for PS exposure upon the cell membrane, and used for detection of apoptotic cells both in vivo and in vitro. Large-scale production of recombinant human annexin V is worth optimization, because of its wide use in nuclear medicine, radiolabeled with (99m)Tc, for the evaluation of cancer chemotherapy treatments, and its use in identification of apoptotic cells in histologic studies. Here we describe the high-yield production of a tag-free version of human annexin V recombinant protein by linear fed-batch cultivation in a bioreactor. |
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