Title |
A ddRAD-based genetic map and its integration with the genome assembly of Japanese eel (Anguilla japonica) provides insights into genome evolution after the teleost-specific genome duplication
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Published in |
BMC Genomics, March 2014
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DOI | 10.1186/1471-2164-15-233 |
Pubmed ID | |
Authors |
Wataru Kai, Kazuharu Nomura, Atushi Fujiwara, Yoji Nakamura, Motoshige Yasuike, Nobuhiko Ojima, Tetsuji Masaoka, Akiyuki Ozaki, Yukinori Kazeto, Koichiro Gen, Jiro Nagao, Hideki Tanaka, Takanori Kobayashi, Mitsuru Ototake |
Abstract |
Recent advancements in next-generation sequencing technology have enabled cost-effective sequencing of whole or partial genomes, permitting the discovery and characterization of molecular polymorphisms. Double-digest restriction-site associated DNA sequencing (ddRAD-seq) is a powerful and inexpensive approach to developing numerous single nucleotide polymorphism (SNP) markers and constructing a high-density genetic map. To enrich genomic resources for Japanese eel (Anguilla japonica), we constructed a ddRAD-based genetic map using an Ion Torrent Personal Genome Machine and anchored scaffolds of the current genome assembly to 19 linkage groups of the Japanese eel. Furthermore, we compared the Japanese eel genome with genomes of model fishes to infer the history of genome evolution after the teleost-specific genome duplication. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Sweden | 2 | 1% |
Portugal | 1 | <1% |
Norway | 1 | <1% |
Brazil | 1 | <1% |
Mexico | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 176 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 49 | 27% |
Researcher | 40 | 22% |
Student > Master | 25 | 14% |
Student > Doctoral Student | 14 | 8% |
Student > Bachelor | 12 | 7% |
Other | 25 | 14% |
Unknown | 19 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 116 | 63% |
Biochemistry, Genetics and Molecular Biology | 27 | 15% |
Environmental Science | 6 | 3% |
Medicine and Dentistry | 4 | 2% |
Earth and Planetary Sciences | 2 | 1% |
Other | 3 | 2% |
Unknown | 26 | 14% |