Title |
Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis
|
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Published in |
BMC Genomics, May 2014
|
DOI | 10.1186/1471-2164-15-381 |
Pubmed ID | |
Authors |
Silvia von der Heyde, Carolin Fromm-Dornieden, Gabriela Salinas-Riester, Tim Beissbarth, Bernhard G Baumgartner |
Abstract |
Adipogenesis is a complex process, in which immature pre-adipocytes change morphology, micro-anatomy and physiology to become mature adipocytes. These store and accumulate fat and release diverse hormones. Massive changes in protein content and protein composition of the transforming cell take place within a short time-frame.In a previous study we analyzed changes in the abundance of free and polysomal, i.e. ribosome bound, RNAs in the first hours of adipogenesis in the murine cell line 3T3-L1. Here we analyze changes of mRNA levels and their potential contribution to the changing protein pool by determination of mRNA levels and ribosome binding to mRNAs in 3T3-L1 cells stimulated for adipogenesis. We grouped mRNA species into categories with respect to up- or down-regulated transcription and translation and analyzed the groups regarding specific functionalities based on Gene Ontology (GO). |
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