Title |
Agrobacterium-derived cytokinin influences plastid morphology and starch accumulation in Nicotiana benthamiana during transient assays
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Published in |
BMC Plant Biology, May 2014
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DOI | 10.1186/1471-2229-14-127 |
Pubmed ID | |
Authors |
Jessica L Erickson, Jörg Ziegler, David Guevara, Steffen Abel, Ralf B Klösgen, Jaideep Mathur, Steven J Rothstein, Martin H Schattat |
Abstract |
Agrobacterium tumefaciens-based transient assays have become a common tool for answering questions related to protein localization and gene expression in a cellular context. The use of these assays assumes that the transiently transformed cells are observed under relatively authentic physiological conditions and maintain 'normal' sub-cellular behaviour. Although this premise is widely accepted, the question of whether cellular organization and organelle morphology is altered in Agrobacterium-infiltrated cells has not been examined in detail. The first indications of an altered sub-cellular environment came from our observation that a common laboratory strain, GV3101(pMP90), caused a drastic increase in stromule frequency. Stromules, or 'stroma-filled-tubules' emanate from the surface of plastids and are sensitive to a variety of biotic and abiotic stresses. Starting from this observation, the goal of our experiments was to further characterize the changes to the cell resulting from short-term bacterial infestation, and to identify the factor responsible for eliciting these changes. |
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Geographical breakdown
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Demographic breakdown
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