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More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection

Overview of attention for article published in BMC Microbiology, May 2014
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Title
More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection
Published in
BMC Microbiology, May 2014
DOI 10.1186/1471-2180-14-121
Pubmed ID
Authors

Daniela I Schneider, Lisa Klasson, Anders E Lind, Wolfgang J Miller

Abstract

Detecting intracellular bacterial symbionts can be challenging when they persist at very low densities. Wolbachia, a widespread bacterial endosymbiont of invertebrates, is particularly challenging. Although it persists at high titers in many species, in others its densities are far below the detection limit of classic end-point Polymerase Chain Reaction (PCR). These low-titer infections can be reliably detected by combining PCR with DNA hybridization, but less elaborate strategies based on end-point PCR alone have proven less sensitive or less general.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 6%
Brazil 2 3%
Italy 1 2%
Czechia 1 2%
Unknown 57 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 29%
Researcher 15 23%
Professor 7 11%
Student > Doctoral Student 4 6%
Student > Master 4 6%
Other 11 17%
Unknown 5 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 60%
Biochemistry, Genetics and Molecular Biology 13 20%
Unspecified 2 3%
Environmental Science 1 2%
Social Sciences 1 2%
Other 2 3%
Unknown 7 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2014.
All research outputs
#18,838,150
of 23,343,453 outputs
Outputs from BMC Microbiology
#2,280
of 3,239 outputs
Outputs of similar age
#165,660
of 228,570 outputs
Outputs of similar age from BMC Microbiology
#35
of 47 outputs
Altmetric has tracked 23,343,453 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,239 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,570 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.