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High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Overview of attention for article published in Microbial Cell Factories, April 2013
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Citations

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164 Mendeley
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Title
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
Published in
Microbial Cell Factories, April 2013
DOI 10.1186/1475-2859-12-37
Pubmed ID
Authors

Hervé Nozach, Carole Fruchart-Gaillard, François Fenaille, Fabrice Beau, Oscar Henrique Pereira Ramos, Badreddine Douzi, Natalie J Saez, Mireille Moutiez, Denis Servent, Muriel Gondry, Robert Thaï, Philippe Cuniasse, Renaud Vincentelli, Vincent Dive

Abstract

Disulfide-rich proteins or DRPs are versatile bioactive compounds that encompass a wide variety of pharmacological, therapeutic, and/or biotechnological applications. Still, the production of DRPs in sufficient quantities is a major bottleneck for their complete structural or functional characterization. Recombinant expression of such small proteins containing multiple disulfide bonds in the bacteria E. coli is considered difficult and general methods and protocols, particularly on a high throughput scale, are limited.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 164 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 1%
Portugal 1 <1%
Germany 1 <1%
Uruguay 1 <1%
Spain 1 <1%
Japan 1 <1%
United States 1 <1%
Unknown 156 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 42 26%
Student > Ph. D. Student 36 22%
Student > Master 20 12%
Student > Bachelor 10 6%
Student > Postgraduate 7 4%
Other 14 9%
Unknown 35 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 55 34%
Agricultural and Biological Sciences 53 32%
Chemistry 9 5%
Pharmacology, Toxicology and Pharmaceutical Science 3 2%
Chemical Engineering 2 1%
Other 8 5%
Unknown 34 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2014.
All research outputs
#17,286,645
of 25,374,917 outputs
Outputs from Microbial Cell Factories
#1,147
of 1,823 outputs
Outputs of similar age
#133,435
of 208,646 outputs
Outputs of similar age from Microbial Cell Factories
#11
of 16 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,823 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 208,646 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.