Title |
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
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Published in |
Microbial Cell Factories, April 2013
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DOI | 10.1186/1475-2859-12-37 |
Pubmed ID | |
Authors |
Hervé Nozach, Carole Fruchart-Gaillard, François Fenaille, Fabrice Beau, Oscar Henrique Pereira Ramos, Badreddine Douzi, Natalie J Saez, Mireille Moutiez, Denis Servent, Muriel Gondry, Robert Thaï, Philippe Cuniasse, Renaud Vincentelli, Vincent Dive |
Abstract |
Disulfide-rich proteins or DRPs are versatile bioactive compounds that encompass a wide variety of pharmacological, therapeutic, and/or biotechnological applications. Still, the production of DRPs in sufficient quantities is a major bottleneck for their complete structural or functional characterization. Recombinant expression of such small proteins containing multiple disulfide bonds in the bacteria E. coli is considered difficult and general methods and protocols, particularly on a high throughput scale, are limited. |
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Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 1% |
Portugal | 1 | <1% |
Germany | 1 | <1% |
Uruguay | 1 | <1% |
Spain | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 156 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 42 | 26% |
Student > Ph. D. Student | 36 | 22% |
Student > Master | 20 | 12% |
Student > Bachelor | 10 | 6% |
Student > Postgraduate | 7 | 4% |
Other | 14 | 9% |
Unknown | 35 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 55 | 34% |
Agricultural and Biological Sciences | 53 | 32% |
Chemistry | 9 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Chemical Engineering | 2 | 1% |
Other | 8 | 5% |
Unknown | 34 | 21% |