Title |
Application of circular consensus sequencing and network analysis to characterize the bovine IgG repertoire
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Published in |
BMC Immunology, September 2012
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DOI | 10.1186/1471-2172-13-52 |
Pubmed ID | |
Authors |
Peter A Larsen, Timothy P L Smith |
Abstract |
Vertebrate immune systems generate diverse repertoires of antibodies capable of mediating response to a variety of antigens. Next generation sequencing methods provide unique approaches to a number of immuno-based research areas including antibody discovery and engineering, disease surveillance, and host immune response to vaccines. In particular, single-molecule circular consensus sequencing permits the sequencing of antibody repertoires at previously unattainable depths of coverage and accuracy. We approached the bovine immunoglobulin G (IgG) repertoire with the objective of characterizing diversity of expressed IgG transcripts. Here we present single-molecule real-time sequencing data of expressed IgG heavy-chain repertoires of four individual cattle. We describe the diversity observed within antigen binding regions and visualize this diversity using a network-based approach. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 4% |
United Kingdom | 1 | 1% |
Netherlands | 1 | 1% |
Spain | 1 | 1% |
China | 1 | 1% |
Unknown | 76 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 28 | 34% |
Student > Ph. D. Student | 18 | 22% |
Student > Master | 7 | 8% |
Other | 5 | 6% |
Professor > Associate Professor | 5 | 6% |
Other | 11 | 13% |
Unknown | 9 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 39 | 47% |
Biochemistry, Genetics and Molecular Biology | 15 | 18% |
Immunology and Microbiology | 5 | 6% |
Veterinary Science and Veterinary Medicine | 3 | 4% |
Chemistry | 3 | 4% |
Other | 8 | 10% |
Unknown | 10 | 12% |