Title |
Whole genome profiling of spontaneous and chemically induced mutations in Toxoplasma gondii
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Published in |
BMC Genomics, May 2014
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DOI | 10.1186/1471-2164-15-354 |
Pubmed ID | |
Authors |
Andrew Farrell, Bradley I Coleman, Brian Benenati, Kevin M Brown, Ira J Blader, Gabor T Marth, Marc-Jan Gubbels |
Abstract |
Next generation sequencing is helping to overcome limitations in organisms less accessible to classical or reverse genetic methods by facilitating whole genome mutational analysis studies. One traditionally intractable group, the Apicomplexa, contains several important pathogenic protozoan parasites, including the Plasmodium species that cause malaria.Here we apply whole genome analysis methods to the relatively accessible model apicomplexan, Toxoplasma gondii, to optimize forward genetic methods for chemical mutagenesis using N-ethyl-N-nitrosourea (ENU) and ethylmethane sulfonate (EMS) at varying dosages. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 2% |
Unknown | 63 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 31% |
Researcher | 12 | 19% |
Student > Bachelor | 6 | 9% |
Student > Master | 6 | 9% |
Student > Postgraduate | 4 | 6% |
Other | 11 | 17% |
Unknown | 5 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 41% |
Biochemistry, Genetics and Molecular Biology | 16 | 25% |
Medicine and Dentistry | 4 | 6% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Business, Management and Accounting | 1 | 2% |
Other | 5 | 8% |
Unknown | 10 | 16% |