Title |
Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China
|
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Published in |
Virology Journal, August 2017
|
DOI | 10.1186/s12985-017-0823-4 |
Pubmed ID | |
Authors |
Huabo Zhou, Lin Liu, Ruikai Li, Yifeng Qin, Qingli Fang, Vinod RMT Balasubramaniam, Guojun Wang, Zuzhang Wei, Kang Ouyang, Weijian Huang, Ying Chen |
Abstract |
Astroviruses (AstVs) have been reported to infect and cause gastroenteritis in most animal species. Human AstVs were regarded the causative agent of viral diarrhea in children. In dogs, little is known about the epidemiology and clinical significance of AstV infection. In this study, we collected and tested 253 rectal swabs from pet dogs; of which 64 samples (25.3%) tested positive for AstVs with diarrhea and 15 more samples (5.9%) also was identified as AstVs, however without any clinical signs. Phylogenetic analysis of 39 partial ORF1b sequences from these samples revealed that they are similar to AstVs, which can be subdivided into three lineages. Interestingly, out of the 39 isolates sequenced, 16 isolates are shown to be in the Mamastrovirus 5/canine astrovirus (CAstV) lineage and the remaining 23 isolates displayed higher similarities with known porcine astrovirus (PoAstV) 5 and 2. Further, analysis of 13 capsid sequences from these isolates showed that they are closely clustered with Chinese or Italy CAstV isolates. The findings indicate that CAstVs commonly circulate in pet dogs, and our sequencing results have shown the genomic diversity of CAstVs leading to increasing number of clusters. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 17% |
Researcher | 3 | 13% |
Other | 1 | 4% |
Lecturer | 1 | 4% |
Student > Doctoral Student | 1 | 4% |
Other | 5 | 22% |
Unknown | 8 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 3 | 13% |
Medicine and Dentistry | 3 | 13% |
Computer Science | 2 | 9% |
Agricultural and Biological Sciences | 2 | 9% |
Unspecified | 1 | 4% |
Other | 2 | 9% |
Unknown | 10 | 43% |