Title |
An integrated platform for bovine DNA methylome analysis suitable for small samples
|
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Published in |
BMC Genomics, June 2014
|
DOI | 10.1186/1471-2164-15-451 |
Pubmed ID | |
Authors |
Habib A Shojaei Saadi, Alan M O’Doherty, Dominic Gagné, Éric Fournier, Jason R Grant, Marc-André Sirard, Claude Robert |
Abstract |
Oocytes and early embryos contain minute amounts of DNA, RNA and proteins, making the study of early mammalian development highly challenging. The study of the embryo epigenome, in particular the DNA methylome, has been made accessible thanks to the possibility of amplifying specific sequences according to their initial methylation status. This paper describes a novel platform dedicated to the genome-wide study of bovine DNA methylation, including a complete pipeline for data analysis and visualization. The platform allows processing and integrating of DNA methylome and transcriptome data from the same sample. Procedures were optimized for genome-wide analysis of 10 ng of DNA (10 bovine blastocysts). Bovine sperm and blastocysts were compared as a test of platform capability. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 2 | 50% |
United States | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 70 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 20 | 28% |
Student > Ph. D. Student | 6 | 8% |
Student > Doctoral Student | 6 | 8% |
Student > Bachelor | 6 | 8% |
Professor > Associate Professor | 5 | 7% |
Other | 15 | 21% |
Unknown | 14 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 43% |
Biochemistry, Genetics and Molecular Biology | 13 | 18% |
Engineering | 3 | 4% |
Nursing and Health Professions | 2 | 3% |
Computer Science | 2 | 3% |
Other | 6 | 8% |
Unknown | 15 | 21% |