Title |
Integration of HIV-1 DNA Is Regulated by Interplay between Viral Rev and Cellular LEDGF/p75 Proteins
|
---|---|
Published in |
Molecular Medicine, October 2009
|
DOI | 10.2119/molmed.2009.00133 |
Pubmed ID | |
Authors |
Aviad Levin, Joseph Rosenbluh, Zvi Hayouka, Assaf Friedler, Abraham Loyter |
Abstract |
The present work describes a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Rev protein and the cellular lens epithelium-derived growth factor p75 (LEDGF/p75) protein in vitro and in virus-infected cells. Here we show, for the first time, that formation of an Rev-LEDGF/p75 complex is a crucial step in regulating viral cDNA integration. Coimmunoprecipitation experiments at various times after virus infection revealed that, first, an integrase enzyme (IN)-LEDGF/p75 complex is formed, which is then replaced by a Rev-LEDGF/p75 and Rev-IN complexes. This was supported by in vitro experiments showing that Rev promotes dissociation of the IN-LEDGF/p75 complex. Combination of the viral IN and the cellular LEDGF/p75 is required for proper integration of the viral cDNA into the host chromosomal DNA. Our findings demonstrate that integration of HIV-1 cDNA is regulated by an interplay between viral Rev and the host-cell LEDGF/p75 proteins. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 9% |
Canada | 1 | 5% |
Unknown | 19 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 27% |
Researcher | 5 | 23% |
Professor | 2 | 9% |
Student > Bachelor | 2 | 9% |
Student > Master | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 27% |
Chemistry | 4 | 18% |
Nursing and Health Professions | 1 | 5% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Medicine and Dentistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 36% |