You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens
|
|---|---|
| Published in |
Nutrition Journal, May 2010
|
| DOI | 10.1186/1475-2891-9-23 |
| Pubmed ID | |
| Authors |
Merlin W Ariefdjohan, Dennis A Savaiano, Cindy H Nakatsu |
| Abstract |
The influence of diet on intestinal microflora has been investigated mainly using conventional microbiological approaches. Although these studies have advanced knowledge on human intestinal microflora, it is imperative that new methods are applied to facilitate scientific progress. Culture-independent molecular fingerprinting method of Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) has been used to study microbial communities in a variety of environmental samples. However, these protocols must be optimized prior to their application in order to enhance the quality and accuracy of downstream analyses. In this study, the relative efficacy of four commercial DNA extraction kits (Mobio Ultra Clean(R) Fecal DNA Isolation Kit, M; QIAamp DNA Stool Mini Kit, Q; FastDNA SPIN Kit, FSp; FastDNA SPIN Kit for Soil, FSo) were evaluated. Further, PCR-DGGE technique was also assessed for its feasibility in detecting differences in human intestinal bacterial fingerprint profiles. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 279 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 1% |
| Germany | 2 | <1% |
| Brazil | 2 | <1% |
| Indonesia | 1 | <1% |
| France | 1 | <1% |
| Colombia | 1 | <1% |
| Canada | 1 | <1% |
| New Zealand | 1 | <1% |
| Slovenia | 1 | <1% |
| Other | 4 | 1% |
| Unknown | 262 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 58 | 21% |
| Researcher | 56 | 20% |
| Student > Master | 51 | 18% |
| Student > Bachelor | 31 | 11% |
| Professor > Associate Professor | 12 | 4% |
| Other | 36 | 13% |
| Unknown | 35 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 131 | 47% |
| Biochemistry, Genetics and Molecular Biology | 32 | 11% |
| Medicine and Dentistry | 19 | 7% |
| Immunology and Microbiology | 17 | 6% |
| Environmental Science | 10 | 4% |
| Other | 25 | 9% |
| Unknown | 45 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2023.
All research outputs
#7,229,557
of 23,577,761 outputs
Outputs from Nutrition Journal
#907
of 1,447 outputs
Outputs of similar age
#32,345
of 96,332 outputs
Outputs of similar age from Nutrition Journal
#5
of 7 outputs
Altmetric has tracked 23,577,761 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 1,447 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 37.2. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 96,332 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.