Title |
Use of chemostat cultures mimicking different phases of wine fermentations as a tool for quantitative physiological analysis
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Published in |
Microbial Cell Factories, June 2014
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DOI | 10.1186/1475-2859-13-85 |
Pubmed ID | |
Authors |
Felícitas Vázquez-Lima, Paulina Silva, Antonio Barreiro, Rubén Martínez-Moreno, Pilar Morales, Manuel Quirós, Ramón González, Joan Albiol, Pau Ferrer |
Abstract |
Saccharomyces cerevisiae is the most relevant yeast species conducting the alcoholic fermentation that takes place during winemaking. Although the physiology of this model organism has been extensively studied, systematic quantitative physiology studies of this yeast under winemaking conditions are still scarce, thus limiting the understanding of fermentative metabolism of wine yeast strains and the systematic description, modelling and prediction of fermentation processes. In this study, we implemented and validated the use of chemostat cultures as a tool to simulate different stages of a standard wine fermentation, thereby allowing to implement metabolic flux analyses describing the sequence of metabolic states of S. cerevisae along the wine fermentation. |
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Mendeley readers
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