Title |
Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole
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Published in |
BMC Microbiology, June 2014
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DOI | 10.1186/1471-2180-14-155 |
Pubmed ID | |
Authors |
Isabel Faria-Ramos, Sofia Farinha, João Neves-Maia, Pedro Ribeiro Tavares, Isabel M Miranda, Letícia M Estevinho, Cidália Pina-Vaz, Acácio G Rodrigues |
Abstract |
The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatus was performed according to the CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation of susceptible A. fumigatus isolates, at 35oC and 180 rpm, in fresh GYEP broth medium supplemented with Prochloraz (PCZ), a potent agricultural antifungal, for a period of 30 days. Minimal inhibitory concentrations (MIC) of PCZ and clinical azoles were monitored every ten days. In order to assess the stability of the developed MIC, the strains were afterwards sub-cultured for an additional 30 days in the absence of antifungal. Along the in vitro induction process, microscopic and macroscopic cultural observations were registered. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Colombia | 1 | 25% |
United Kingdom | 1 | 25% |
Venezuela, Bolivarian Republic of | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 1% |
Denmark | 1 | 1% |
Unknown | 74 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 18% |
Student > Bachelor | 10 | 13% |
Researcher | 10 | 13% |
Student > Master | 9 | 12% |
Student > Doctoral Student | 7 | 9% |
Other | 14 | 18% |
Unknown | 12 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 14 | 18% |
Immunology and Microbiology | 12 | 16% |
Agricultural and Biological Sciences | 12 | 16% |
Medicine and Dentistry | 6 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
Other | 7 | 9% |
Unknown | 20 | 26% |