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Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma

Overview of attention for article published in BMC Genomics, June 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

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9 X users
patent
1 patent
googleplus
1 Google+ user

Citations

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65 Dimensions

Readers on

mendeley
116 Mendeley
citeulike
4 CiteULike
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Title
Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma
Published in
BMC Genomics, June 2014
DOI 10.1186/1471-2164-15-476
Pubmed ID
Authors

Kristina Warton, Vita Lin, Tina Navin, Nicola J Armstrong, Warren Kaplan, Kevin Ying, Brian Gloss, Helena Mangs, Shalima S Nair, Neville F Hacker, Robert L Sutherland, Susan J Clark, Goli Samimi

Abstract

Free circulating DNA (fcDNA) has many potential clinical applications, due to the non-invasive way in which it is collected. However, because of the low concentration of fcDNA in blood, genome-wide analysis carries many technical challenges that must be overcome before fcDNA studies can reach their full potential. There are currently no definitive standards for fcDNA collection, processing and whole-genome sequencing. We report novel detailed methodology for the capture of high-quality methylated fcDNA, library preparation and downstream genome-wide Next-Generation Sequencing. We also describe the effects of sample storage, processing and scaling on fcDNA recovery and quality.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
Netherlands 1 <1%
Germany 1 <1%
China 1 <1%
Chile 1 <1%
Unknown 109 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 37 32%
Student > Ph. D. Student 25 22%
Other 14 12%
Student > Master 8 7%
Lecturer 4 3%
Other 13 11%
Unknown 15 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 33 28%
Agricultural and Biological Sciences 32 28%
Medicine and Dentistry 19 16%
Engineering 4 3%
Computer Science 2 2%
Other 5 4%
Unknown 21 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2015.
All research outputs
#3,641,636
of 23,295,606 outputs
Outputs from BMC Genomics
#1,348
of 10,739 outputs
Outputs of similar age
#35,939
of 229,238 outputs
Outputs of similar age from BMC Genomics
#20
of 204 outputs
Altmetric has tracked 23,295,606 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,739 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 229,238 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 204 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.