Title |
A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome
|
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Published in |
BMC Genomics, June 2014
|
DOI | 10.1186/1471-2164-15-518 |
Pubmed ID | |
Authors |
Željko M Radulović, Tae K Kim, Lindsay M Porter, Sing-Hoi Sze, Lauren Lewis, Albert Mulenga |
Abstract |
Multiple tick saliva proteins, the majority of which are unknown, confer tick resistance in repeatedly infested animals. The objective of this study was to identify the 24-48 h fed Amblyomma americanum tick saliva immuno-proteome. The 24-48 h tick-feeding phase is critical to tick parasitism as it precedes important events in tick biology, blood meal feeding and disease agent transmission. Fed male, 24 and 96 h fed female phage display cDNA expression libraries were biopanned using rabbit antibodies to 24 and 48 h fed A. americanum female tick saliva proteins. Biopanned immuno-cDNA libraries were subjected to next generation sequencing, de novo assembly, and bioinformatic analysis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Indonesia | 1 | 1% |
Colombia | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 89 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 22% |
Researcher | 13 | 14% |
Student > Master | 13 | 14% |
Student > Doctoral Student | 8 | 9% |
Student > Bachelor | 6 | 7% |
Other | 12 | 13% |
Unknown | 20 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 32 | 35% |
Biochemistry, Genetics and Molecular Biology | 13 | 14% |
Veterinary Science and Veterinary Medicine | 8 | 9% |
Chemistry | 3 | 3% |
Medicine and Dentistry | 2 | 2% |
Other | 7 | 8% |
Unknown | 27 | 29% |