Title |
A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat
|
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Published in |
BMC Plant Biology, August 2009
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DOI | 10.1186/1471-2229-9-115 |
Pubmed ID | |
Authors |
Cristobal Uauy, Francine Paraiso, Pasqualina Colasuonno, Robert K Tran, Helen Tsai, Steve Berardi, Luca Comai, Jorge Dubcovsky |
Abstract |
Wheat (Triticum ssp.) is an important food source for humans in many regions around the world. However, the ability to understand and modify gene function for crop improvement is hindered by the lack of available genomic resources. TILLING is a powerful reverse genetics approach that combines chemical mutagenesis with a high-throughput screen for mutations. Wheat is specially well-suited for TILLING due to the high mutation densities tolerated by polyploids, which allow for very efficient screens. Despite this, few TILLING populations are currently available. In addition, current TILLING screening protocols require high-throughput genotyping platforms, limiting their use. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Australia | 3 | 1% |
United States | 2 | <1% |
United Kingdom | 2 | <1% |
France | 1 | <1% |
Sweden | 1 | <1% |
Turkey | 1 | <1% |
Canada | 1 | <1% |
New Zealand | 1 | <1% |
India | 1 | <1% |
Other | 4 | 1% |
Unknown | 264 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 91 | 32% |
Student > Ph. D. Student | 72 | 26% |
Student > Master | 23 | 8% |
Student > Bachelor | 17 | 6% |
Professor | 12 | 4% |
Other | 42 | 15% |
Unknown | 24 | 9% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 217 | 77% |
Biochemistry, Genetics and Molecular Biology | 25 | 9% |
Computer Science | 3 | 1% |
Chemistry | 2 | <1% |
Medicine and Dentistry | 2 | <1% |
Other | 4 | 1% |
Unknown | 28 | 10% |