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Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Overview of attention for article published in Journal of Animal Science and Biotechnology, September 2017
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Title
Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study
Published in
Journal of Animal Science and Biotechnology, September 2017
DOI 10.1186/s40104-017-0201-5
Pubmed ID
Authors

Suman Choudhary, A. Kumaresan, Manish Kumar, Shivani Chhillar, Hrudananda Malik, Sudarshan Kumar, Jai K. Kaushik, Tirtha K. Datta, Ashok K. Mohanty

Abstract

An oviduct- specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen- thawed sperm functions and in vitro embryo development. The proportion of viable sperms was greater (P < 0.05) in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at 3 h and 4 h of incubation; and membrane- intact sperms at 4 h was greater (P < 0.05) in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome- reacted sperms was greater (P < 0.05) in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater (P < 0.05) in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL. The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence, our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 29%
Student > Ph. D. Student 1 14%
Other 1 14%
Student > Bachelor 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Psychology 1 14%
Unknown 4 57%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2017.
All research outputs
#10,044,426
of 12,554,960 outputs
Outputs from Journal of Animal Science and Biotechnology
#219
of 273 outputs
Outputs of similar age
#194,913
of 264,715 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#5
of 5 outputs
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