Title |
Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
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Published in |
BMC Veterinary Research, June 2014
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DOI | 10.1186/1746-6148-10-125 |
Pubmed ID | |
Authors |
Martina Stirn, Andreas Moritz, Natali Bauer |
Abstract |
Modern automated haematology instruments are capable of performing leukocyte differentials faster, cheaper and with a higher precision than the traditional 100-cell manual differential count. Thus, in human laboratories, criteria are defined for performing a manual review of the blood smear resulting in a marked reduction of manual differential counts. While common in human laboratories, this approach to reducing the number of manual differentials in veterinary laboratories is still not commonly performed. Thus, our aim was to determine the rate and causes of manual leukocyte differentials in a university clinical pathology laboratory using the automated laser-based haematology analyser ADVIA 120. Overall, 14,953 complete blood cell counts from dogs, cats and horses were reviewed. Manual leukocyte differentials were requested if abnormal ADVIA peroxidase and baso cytograms were detected (i.e. suspicion of left shift or atypical lymphocytes/blasts, inappropriate separation of cell populations). |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 3% |
Unknown | 35 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 8 | 22% |
Professor > Associate Professor | 5 | 14% |
Student > Bachelor | 4 | 11% |
Researcher | 4 | 11% |
Other | 3 | 8% |
Other | 5 | 14% |
Unknown | 7 | 19% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 15 | 42% |
Medicine and Dentistry | 6 | 17% |
Agricultural and Biological Sciences | 4 | 11% |
Social Sciences | 1 | 3% |
Chemistry | 1 | 3% |
Other | 1 | 3% |
Unknown | 8 | 22% |