Title |
Antifungal activity of synthetic naphthoquinones against dermatophytes and opportunistic fungi: preliminary mechanism-of-action tests
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Published in |
Annals of Clinical Microbiology and Antimicrobials, July 2014
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DOI | 10.1186/1476-0711-13-26 |
Pubmed ID | |
Authors |
Maria do Perpetuo Socorro Borges Carriço Ferreira, Mariana Filomena do Carmo Cardoso, Fernando de Carvalho da Silva, Vitor Francisco Ferreira, Emerson Silva Lima, João Vicente Braga Souza |
Abstract |
This study evaluated the antifungal activities of synthetic naphthoquinones against opportunistic and dermatophytic fungi and their preliminary mechanisms of action. The minimum inhibitory concentrations (MICs) of four synthetic naphthoquinones for 89 microorganisms, including opportunistic yeast agents, dermatophytes and opportunistic filamentous fungi, were determined. The compound that exhibited the best activity was assessed for its action against the cell wall (sorbitol test), for interference associated with ergosterol interaction, for osmotic balance (K+ efflux) and for membrane leakage of substances that absorb at the wavelength of 260 nm. All tested naphthoquinones exhibited antifungal activity, and compound IVS320 (3a,10b-dihydro-1H-cyclopenta [b] naphtho [2,3-d] furan-5,10-dione)-dione) demonstrated the lowest MICs across the tested species. The MIC of IVS320 was particularly low for dermatophytes (values ranging from 5-28 mug/mL) and Cryptococcus spp. (3-5 mug/mL). In preliminary mechanism-of-action tests, IVS320 did not alter the fungal cell wall but did cause problems in terms of cell membrane permeability (efflux of K+ and leakage of substances that absorb at 260 nm). This last effect was unrelated to ergosterol interactions with the membrane. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 50% |
Venezuela, Bolivarian Republic of | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 8 | 14% |
Student > Bachelor | 7 | 12% |
Student > Doctoral Student | 5 | 9% |
Student > Ph. D. Student | 5 | 9% |
Researcher | 4 | 7% |
Other | 12 | 21% |
Unknown | 16 | 28% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 11 | 19% |
Medicine and Dentistry | 9 | 16% |
Pharmacology, Toxicology and Pharmaceutical Science | 7 | 12% |
Biochemistry, Genetics and Molecular Biology | 4 | 7% |
Chemistry | 3 | 5% |
Other | 6 | 11% |
Unknown | 17 | 30% |