Title |
Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture
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Published in |
Proteome Science, July 2014
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DOI | 10.1186/1477-5956-12-39 |
Pubmed ID | |
Authors |
Uros Rajcevic, Jaco C Knol, Sander Piersma, Sébastien Bougnaud, Fred Fack, Eirik Sundlisaeter, Karl Søndenaa, Reidar Myklebust, Thang V Pham, Simone P Niclou, Connie R Jiménez |
Abstract |
Organotypic tumor spheroids, a 3D in vitro model derived from patient tumor material, preserve tissue heterogeneity and retain structural tissue elements, thus replicating the in vivo tumor more closely than commonly used 2D and 3D cell line models. Such structures harbour tumorigenic cells, as revealed by xenograft implantation studies in animal models and maintain the genetic makeup of the original tumor material. The aim of our work was a morphological and proteomic characterization of organotypic spheroids derived from colorectal cancer tissue in order to get insight into their composition and associated biology. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Germany | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Iran, Islamic Republic of | 1 | <1% |
United Kingdom | 1 | <1% |
France | 1 | <1% |
Luxembourg | 1 | <1% |
Unknown | 112 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 35 | 30% |
Researcher | 25 | 22% |
Professor | 9 | 8% |
Student > Bachelor | 9 | 8% |
Student > Doctoral Student | 8 | 7% |
Other | 17 | 15% |
Unknown | 13 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 25 | 22% |
Agricultural and Biological Sciences | 21 | 18% |
Medicine and Dentistry | 11 | 9% |
Engineering | 9 | 8% |
Chemistry | 8 | 7% |
Other | 22 | 19% |
Unknown | 20 | 17% |