Title |
Cross-section perimeter is a suitable parameter to describe the effects of different baffle geometries in shaken microtiter plates
|
---|---|
Published in |
Journal of Biological Engineering, July 2014
|
DOI | 10.1186/1754-1611-8-18 |
Pubmed ID | |
Authors |
Clemens Lattermann, Matthias Funke, Sven Hansen, Sylvia Diederichs, Jochen Büchs |
Abstract |
Biotechnological screening processes are performed since more than 8 decades in small scale shaken bioreactors like shake flasks or microtiter plates. One of the major issues of such reactors is the sufficient oxygen supply of suspended microorganisms. Oxygen transfer into the bulk liquid can in general be increased by introducing suitable baffles at the reactor wall. However, a comprehensive and systematic characterization of baffled shaken bioreactors has never been carried out so far. Baffles often differ in number, size and shape. The exact geometry of baffles in glass lab ware like shake flasks is very difficult to reproduce from piece to piece due to the hard to control flow behavior of molten glass during manufacturing. Thus, reproducibility of the maximum oxygen transfer capacity in such baffled shake flasks is hardly given. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Unknown | 42 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 26% |
Student > Ph. D. Student | 8 | 19% |
Student > Master | 7 | 16% |
Other | 4 | 9% |
Student > Bachelor | 2 | 5% |
Other | 6 | 14% |
Unknown | 5 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 28% |
Biochemistry, Genetics and Molecular Biology | 11 | 26% |
Engineering | 10 | 23% |
Chemical Engineering | 2 | 5% |
Chemistry | 1 | 2% |
Other | 0 | 0% |
Unknown | 7 | 16% |