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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Development of a two-step cultivation strategy for the production of vitamin B12 by Bacillus megaterium
|
|---|---|
| Published in |
Microbial Cell Factories, July 2014
|
| DOI | 10.1186/s12934-014-0102-7 |
| Pubmed ID | |
| Authors |
Yousef Mohammed, Byong Lee, Zhen Kang, Guocheng Du |
| Abstract |
BackgroundVitamin B12 is a fascinating molecule which acts as a co-factor in the metabolism of many organisms, especially affecting DNA synthesis and regulation, fatty acid synthesis and energy production. The synthesis of vitamin B12 is limited to a few of bacteria and archaea. Therefore, industrial microbial fermentation is used to meet annual demands worldwide of vitamin B12 and as an alternative method to the chemical synthesis which requires at least 60 steps that is uneconomical. Bacillus megaterium is one of vitamin B12 producers and an ideal host for many biotechnology applications and being one of the best tools for the industrial production of several enzymes. Therefore, a two-step optimization strategy was established to produce high yield of vitamin B12 by B. megaterium through the provision of the production requirements and the suitable conditions for the biosynthesis of vitamin B12.ResultsWe achieved the optimum conditions for the fermentation process of B. megaterium to produce high yield of vitamin B12 in a practical way based on statistical design and analysis which allowed vitamin B12 production to increase up to 759-fold (204.46 ¿g/l) as compared with control without parameters (0.26 ¿g/L). High performance liquid chromatography coupled to variable wavelength detector and mass spectrometry has been used to identify vitamin B12 forms and confirm the results.ConclusionsWe developed the fermentation process of B. megaterium to enhance the production of vitamin B12 by providing the required supplements for the synthesis of vitamin B12 (CoCl2, ¿-aminolevulinic acid (ALA) and 5,6-dimethylbenzimidazole (DMB)) and dividing the fermentation process into three stages. In addition, the optimum incubation times of the three fermentation stages were investigated and performed with reducing number of experimental and evaluated multiple parameters and their interactions by using statistical experimental design and analysis. All of these strategies has proven successful in enhancing the production of vitamin B12 up to 204.46 ¿g/l and demonstrated that B. megaterium could be a good candidate for the industrial production of vitamin B12. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Egypt | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Finland | 1 | <1% |
| Unknown | 115 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 21 | 18% |
| Researcher | 21 | 18% |
| Student > Ph. D. Student | 15 | 13% |
| Student > Master | 13 | 11% |
| Student > Doctoral Student | 1 | <1% |
| Other | 2 | 2% |
| Unknown | 43 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 19% |
| Agricultural and Biological Sciences | 19 | 16% |
| Engineering | 6 | 5% |
| Immunology and Microbiology | 6 | 5% |
| Chemistry | 5 | 4% |
| Other | 10 | 9% |
| Unknown | 48 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2022.
All research outputs
#7,737,702
of 23,532,144 outputs
Outputs from Microbial Cell Factories
#553
of 1,659 outputs
Outputs of similar age
#74,345
of 228,493 outputs
Outputs of similar age from Microbial Cell Factories
#10
of 28 outputs
Altmetric has tracked 23,532,144 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,659 research outputs from this source. They receive a mean Attention Score of 4.6. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,493 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.