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Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris

Overview of attention for article published in BMC Microbiology, November 2013
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Title
Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris
Published in
BMC Microbiology, November 2013
DOI 10.1186/1471-2180-13-250
Pubmed ID
Authors

Jose Antonio Gama Salgado, Martin Kangwa, Marcelo Fernandez-Lahore

Abstract

Extracellular aspartic proteinase (MCAP) produced by Mucor circinelloides in solid state fermentations has been shown to possess milk clotting activity and represents a potential replacement for bovine chymosin in cheese manufacturing. Despite its prospects in the dairy industry, the molecular characteristics of this enzyme remain unknown. This work focuses on MCAP cloning and optimization of heterologous expression in Pichia pastoris, and characterization of the enzyme.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 3%
Unknown 36 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 32%
Student > Ph. D. Student 8 22%
Student > Master 4 11%
Student > Postgraduate 3 8%
Student > Bachelor 2 5%
Other 4 11%
Unknown 4 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 41%
Biochemistry, Genetics and Molecular Biology 9 24%
Engineering 3 8%
Unspecified 1 3%
Arts and Humanities 1 3%
Other 3 8%
Unknown 5 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2014.
All research outputs
#20,233,066
of 22,758,963 outputs
Outputs from BMC Microbiology
#2,685
of 3,184 outputs
Outputs of similar age
#187,353
of 215,075 outputs
Outputs of similar age from BMC Microbiology
#28
of 33 outputs
Altmetric has tracked 22,758,963 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,184 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 215,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.