Title |
mmquant: how to count multi-mapping reads?
|
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Published in |
BMC Bioinformatics, September 2017
|
DOI | 10.1186/s12859-017-1816-4 |
Pubmed ID | |
Authors |
Matthias Zytnicki |
Abstract |
RNA-Seq is currently used routinely, and it provides accurate information on gene transcription. However, the method cannot accurately estimate duplicated genes expression. Several strategies have been previously used (drop duplicated genes, distribute uniformly the reads, or estimate expression), but all of them provide biased results. We provide here a tool, called mmquant, for computing gene expression, included duplicated genes. If a read maps at different positions, the tool detects that the corresponding genes are duplicated; it merges the genes and creates a merged gene. The counts of ambiguous reads is then based on the input genes and the merged genes. mmquant is a drop-in replacement of the widely used tools htseq-count and featureCounts that handles multi-mapping reads in an unabiased way. |
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Geographical breakdown
Country | Count | As % |
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France | 8 | 38% |
United Kingdom | 2 | 10% |
Netherlands | 1 | 5% |
Norway | 1 | 5% |
Italy | 1 | 5% |
Unknown | 8 | 38% |
Demographic breakdown
Type | Count | As % |
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Scientists | 11 | 52% |
Members of the public | 10 | 48% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 115 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 31 | 27% |
Student > Ph. D. Student | 23 | 20% |
Student > Master | 19 | 17% |
Student > Bachelor | 11 | 10% |
Other | 4 | 3% |
Other | 7 | 6% |
Unknown | 20 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 35 | 30% |
Biochemistry, Genetics and Molecular Biology | 34 | 30% |
Computer Science | 9 | 8% |
Medicine and Dentistry | 4 | 3% |
Neuroscience | 2 | 2% |
Other | 6 | 5% |
Unknown | 25 | 22% |