Title |
Polymerase chain reaction–based assays for the diagnosis of human brucellosis
|
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Published in |
Annals of Clinical Microbiology and Antimicrobials, August 2014
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DOI | 10.1186/s12941-014-0031-7 |
Pubmed ID | |
Authors |
Ying Wang, Zhanli Wang, Yaxian Zhang, Liyun Bai, Yue Zhao, Chunfang Liu, An Ma, Hui Yu |
Abstract |
Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | <1% |
Kenya | 1 | <1% |
Norway | 1 | <1% |
Qatar | 1 | <1% |
Unknown | 106 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 20 | 18% |
Researcher | 12 | 11% |
Student > Ph. D. Student | 9 | 8% |
Student > Postgraduate | 8 | 7% |
Student > Bachelor | 7 | 6% |
Other | 29 | 26% |
Unknown | 25 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 28 | 25% |
Veterinary Science and Veterinary Medicine | 18 | 16% |
Medicine and Dentistry | 12 | 11% |
Biochemistry, Genetics and Molecular Biology | 9 | 8% |
Immunology and Microbiology | 9 | 8% |
Other | 4 | 4% |
Unknown | 30 | 27% |