Title |
Phenotypic plasticity in normal breast derived epithelial cells
|
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Published in |
BMC Molecular and Cell Biology, June 2014
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DOI | 10.1186/1471-2121-15-20 |
Pubmed ID | |
Authors |
Candice AM Sauder, Jillian E Koziel, MiRan Choi, Melanie J Fox, Brenda R Grimes, Sunil Badve, Rachel J Blosser, Milan Radovich, Christina C Lam, Melville B Vaughan, Brittney-Shea Herbert, Susan E Clare |
Abstract |
Normal, healthy human breast tissue from a variety of volunteer donors has become available for research thanks to the establishment of the Susan G. Komen for the Cure® Tissue Bank at the IU Simon Cancer Center (KTB). Multiple epithelial (K-HME) and stromal cells (K-HMS) were established from the donated tissue. Explant culture was utilized to isolate the cells from pieces of breast tissue. Selective media and trypsinization were employed to select either epithelial cells or stromal cells. The primary, non-transformed epithelial cells, the focus of this study, were characterized by immunohistochemistry, flow cytometry, and in vitro cell culture. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 4 | 19% |
Student > Master | 3 | 14% |
Student > Ph. D. Student | 3 | 14% |
Librarian | 2 | 10% |
Professor | 2 | 10% |
Other | 4 | 19% |
Unknown | 3 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 29% |
Medicine and Dentistry | 4 | 19% |
Agricultural and Biological Sciences | 3 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
Chemical Engineering | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 19% |