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Recent advances in live cell imaging of hepatoma cells

Overview of attention for article published in BMC Molecular and Cell Biology, July 2014
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Title
Recent advances in live cell imaging of hepatoma cells
Published in
BMC Molecular and Cell Biology, July 2014
DOI 10.1186/1471-2121-15-26
Pubmed ID
Authors

Sandeep Salipalli, Prafull Kumar Singh, Jürgen Borlak

Abstract

Live cell imaging enables the study of dynamic processes of living cells in real time by use of suitable reporter proteins and the staining of specific cellular structures and/or organelles. With the availability of advanced optical devices and improved cell culture protocols it has become a rapidly growing research methodology. The success of this technique relies mainly on the selection of suitable reporter proteins, construction of recombinant plasmids possessing cell type specific promoters as well as reliable methods of gene transfer. This review aims to provide an overview of the recent developments in the field of marker proteins (bioluminescence and fluorescent) and methodologies (fluorescent resonance energy transfer, fluorescent recovery after photobleaching and proximity ligation assay) employed as to achieve an improved imaging of biological processes in hepatoma cells. Moreover, different expression systems of marker proteins and the modes of gene transfer are discussed with emphasis on the study of lipid droplet formation in hepatocytes as an example.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 25%
Student > Ph. D. Student 5 14%
Student > Bachelor 3 8%
Student > Master 3 8%
Professor > Associate Professor 2 6%
Other 5 14%
Unknown 9 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 28%
Chemistry 5 14%
Immunology and Microbiology 4 11%
Biochemistry, Genetics and Molecular Biology 3 8%
Medicine and Dentistry 2 6%
Other 2 6%
Unknown 10 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2014.
All research outputs
#22,778,604
of 25,394,764 outputs
Outputs from BMC Molecular and Cell Biology
#1,054
of 1,233 outputs
Outputs of similar age
#206,685
of 240,438 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#16
of 25 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.