This was shown to also be the case with Phenol based reagents a couple of years ago as well: https://t.co/LZsqnxqcfN RNAlater and flash freezing storage methods nonrandomly influence observed gene expression in RNAseq experiments https://t.co/I8zSC2H9s4
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We show that Qiagen or TRIzol extraction methods retain differentially nuclear RNA species, and that consequently, rRNA depletion-based RNA sequencing protocols are particularly sensitive to the extraction methods. https://t.co/nZphuQx4cc
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Influence of RNA extraction methods and library selection schemes on RNA-seq data http://t.co/NsdZSIVifz #bmcgenomics
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Influence of RNA extraction methods and library selection schemes on RNA-seq data http://t.co/vfV4XpIyA2 #bmcgenomics