Title |
Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan
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Published in |
BMC Microbiology, September 2011
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DOI | 10.1186/1471-2180-11-200 |
Pubmed ID | |
Authors |
Zareen Fatima, Muhammad Idrees, Mohammad A Bajwa, Zarfishan Tahir, Obaid Ullah, Muhammad Q Zia, Abrar Hussain, Madiha Akram, Bushra Khubaib, Samia Afzal, Saira Munir, Sana Saleem, Bisma Rauff, Sadaf Badar, Mahrukh Naudhani, Sadia Butt, Mahwish Aftab, Liaqat Ali, Muhammad Ali |
Abstract |
Since the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have erupted in the region. Dengue virus serotype 3 (DEN-3) was first documented in 2005 outbreak in Karachi. Reports show that serotype 3 is prevalent in Lahore since 2008. Serotype 2 (DEN-2) is the major circulating serotype in Pakistan as it is documented since 1994. We have conducted a detailed study of three outbreaks of dengue virus infection that occurred in years 2007, 2008 and 2009 in Lahore by using molecular techniques such as PCR and nucleotide sequencing of the C-prM gene junction of Dengue virus. |
Mendeley readers
Geographical breakdown
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Malaysia | 2 | 1% |
Colombia | 1 | <1% |
Germany | 1 | <1% |
Indonesia | 1 | <1% |
United Kingdom | 1 | <1% |
Mexico | 1 | <1% |
United States | 1 | <1% |
Unknown | 167 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 28 | 16% |
Researcher | 25 | 14% |
Student > Master | 24 | 14% |
Student > Bachelor | 23 | 13% |
Student > Postgraduate | 13 | 7% |
Other | 28 | 16% |
Unknown | 34 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 48 | 27% |
Medicine and Dentistry | 23 | 13% |
Biochemistry, Genetics and Molecular Biology | 20 | 11% |
Immunology and Microbiology | 19 | 11% |
Nursing and Health Professions | 5 | 3% |
Other | 23 | 13% |
Unknown | 37 | 21% |