Title |
Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
|
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Published in |
Investigative Genetics, August 2014
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DOI | 10.1186/2041-2223-5-10 |
Pubmed ID | |
Authors |
Mindy James, Ulrich Melcher, Jacqueline Fletcher |
Abstract |
Crops in the USA are vulnerable to natural and criminal threats because of their widespread cultivation and lack of surveillance, and because of implementation of growing practices such as monoculture. To prepare for investigation and attribution of such events, forensic assays, including determination of molecular profiles, are being adapted for use with plant pathogens. The use of multi-locus variable number tandem repeat (VNTR) analysis (MLVA) and multi-locus sequence typing (MLST) in investigations involving plant pathogens may be problematic because the long lag periods between pathogen introduction and discovery of associated disease may provide enough time for evolution to occur in the regions of the genome employed in each assay. Thus, more information on the stability of the loci employed in these methods is needed. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 25% |
Researcher | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 38% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Unknown | 3 | 38% |