Title |
Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp
|
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Published in |
BMC Veterinary Research, July 2014
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DOI | 10.1186/1746-6148-10-150 |
Pubmed ID | |
Authors |
Carlos A G Leal, Alex F Carvalho, Rômulo C Leite, Henrique C P Figueiredo |
Abstract |
The White spot syndrome virus (WSSV) and Penaeus stylirostris penstyldensovirus 1 (previously named Infectious hypodermal and hematopoietic necrosis virus-IHHNV) are two of the most important viral pathogens of penaeid shrimp. Different methods have been applied for diagnosis of these viruses, including Real-time PCR (qPCR) assays. A duplex qPCR method allows the simultaneous detection of two viruses in the same sample, which is more cost-effective than assaying for each virus separately. Currently, an assay for the simultaneous detection of the WSSV and the PstDV1 in shrimp is unavailable. The aim of this study was to develop and standardize a duplex qPCR assay for the simultaneous detection of the WSSV and the PstDV1 in clinical samples of diseased L. vannamei. In addition, to evaluate the performance of two qPCR master mixes with regard to the clinical sensitivity of the qPCR assay, as well as, different methods for qPCR results evaluation. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 5 | 15% |
Student > Postgraduate | 4 | 12% |
Student > Ph. D. Student | 3 | 9% |
Researcher | 3 | 9% |
Student > Master | 3 | 9% |
Other | 10 | 29% |
Unknown | 6 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 10 | 29% |
Agricultural and Biological Sciences | 10 | 29% |
Medicine and Dentistry | 3 | 9% |
Social Sciences | 2 | 6% |
Chemistry | 1 | 3% |
Other | 0 | 0% |
Unknown | 8 | 24% |