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Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp

Overview of attention for article published in BMC Veterinary Research, July 2014
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Title
Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp
Published in
BMC Veterinary Research, July 2014
DOI 10.1186/1746-6148-10-150
Pubmed ID
Authors

Carlos A G Leal, Alex F Carvalho, Rômulo C Leite, Henrique C P Figueiredo

Abstract

The White spot syndrome virus (WSSV) and Penaeus stylirostris penstyldensovirus 1 (previously named Infectious hypodermal and hematopoietic necrosis virus-IHHNV) are two of the most important viral pathogens of penaeid shrimp. Different methods have been applied for diagnosis of these viruses, including Real-time PCR (qPCR) assays. A duplex qPCR method allows the simultaneous detection of two viruses in the same sample, which is more cost-effective than assaying for each virus separately. Currently, an assay for the simultaneous detection of the WSSV and the PstDV1 in shrimp is unavailable. The aim of this study was to develop and standardize a duplex qPCR assay for the simultaneous detection of the WSSV and the PstDV1 in clinical samples of diseased L. vannamei. In addition, to evaluate the performance of two qPCR master mixes with regard to the clinical sensitivity of the qPCR assay, as well as, different methods for qPCR results evaluation.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Unknown 33 97%

Demographic breakdown

Readers by professional status Count As %
Other 5 15%
Student > Postgraduate 4 12%
Student > Ph. D. Student 3 9%
Researcher 3 9%
Student > Master 3 9%
Other 10 29%
Unknown 6 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 29%
Agricultural and Biological Sciences 10 29%
Medicine and Dentistry 3 9%
Social Sciences 2 6%
Chemistry 1 3%
Other 0 0%
Unknown 8 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2014.
All research outputs
#18,376,056
of 22,760,687 outputs
Outputs from BMC Veterinary Research
#1,915
of 3,042 outputs
Outputs of similar age
#163,070
of 227,323 outputs
Outputs of similar age from BMC Veterinary Research
#25
of 38 outputs
Altmetric has tracked 22,760,687 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,042 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,323 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.