Title |
Comparison of three methods for detection of gametocytes in Melanesian children treated for uncomplicated malaria
|
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Published in |
Malaria Journal, August 2014
|
DOI | 10.1186/1475-2875-13-319 |
Pubmed ID | |
Authors |
Stephan Karl, Moses Laman, Tamarah Koleala, Clemencia Ibam, Bernadine Kasian, Nola N’Drewei, Anna Rosanas-Urgell, Brioni R Moore, Andreea Waltmann, Cristian Koepfli, Peter M Siba, Inoni Betuela, Robert C Woodward, Timothy G St Pierre, Ivo Mueller, Timothy ME Davis |
Abstract |
Gametocytes are the transmission stages of Plasmodium parasites, the causative agents of malaria. As their density in the human host is typically low, they are often undetected by conventional light microscopy. Furthermore, application of RNA-based molecular detection methods for gametocyte detection remains challenging in remote field settings. In the present study, a detailed comparison of three methods, namely light microscopy, magnetic fractionation and reverse transcriptase polymerase chain reaction for detection of Plasmodium falciparum and Plasmodium vivax gametocytes was conducted. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Madagascar | 2 | 3% |
United States | 2 | 3% |
Brazil | 1 | 1% |
Tanzania, United Republic of | 1 | 1% |
United Kingdom | 1 | 1% |
Unknown | 65 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 19 | 26% |
Researcher | 17 | 24% |
Student > Master | 8 | 11% |
Student > Bachelor | 5 | 7% |
Student > Doctoral Student | 4 | 6% |
Other | 11 | 15% |
Unknown | 8 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 25% |
Medicine and Dentistry | 18 | 25% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Immunology and Microbiology | 2 | 3% |
Physics and Astronomy | 2 | 3% |
Other | 11 | 15% |
Unknown | 14 | 19% |