Title |
Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
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Published in |
Frontiers in Zoology, July 2008
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DOI | 10.1186/1742-9994-5-12 |
Pubmed ID | |
Authors |
Hege Vestheim, Simon N Jarman |
Abstract |
Identification of DNA sequence diversity is a powerful means for assessing the species present in environmental samples. The most common molecular strategies for estimating taxonomic composition depend upon PCR with universal primers that amplify an orthologous DNA region from a range of species. The diversity of sequences within a sample that can be detected by universal primers is often compromised by high concentrations of some DNA templates. If the DNA within the sample contains a small number of sequences in relatively high concentrations, then less concentrated sequences are often not amplified because the PCR favours the dominant DNA types. This is a particular problem in molecular diet studies, where predator DNA is often present in great excess of food-derived DNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 13 | 2% |
Germany | 7 | <1% |
United Kingdom | 4 | <1% |
Chile | 3 | <1% |
Poland | 2 | <1% |
Denmark | 2 | <1% |
Canada | 2 | <1% |
Finland | 2 | <1% |
Sweden | 2 | <1% |
Other | 12 | 1% |
Unknown | 816 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 200 | 23% |
Researcher | 197 | 23% |
Student > Master | 114 | 13% |
Student > Bachelor | 71 | 8% |
Other | 44 | 5% |
Other | 121 | 14% |
Unknown | 118 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 432 | 50% |
Biochemistry, Genetics and Molecular Biology | 118 | 14% |
Environmental Science | 86 | 10% |
Chemistry | 13 | 2% |
Immunology and Microbiology | 12 | 1% |
Other | 60 | 7% |
Unknown | 144 | 17% |