Title |
Characteristics of replication-independent endogenous double-strand breaks in Saccharomyces cerevisiae
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Published in |
BMC Genomics, September 2014
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DOI | 10.1186/1471-2164-15-750 |
Pubmed ID | |
Authors |
Monnat Pongpanich, Maturada Patchsung, Jirapan Thongsroy, Apiwat Mutirangura |
Abstract |
Replication-independent endogenous double-strand breaks (RIND-EDSBs) occur in both humans and yeast in the absence of inductive agents and DNA replication. In human cells, RIND-EDSBs are hypermethylated, preferentially retained in the heterochromatin and unbound by γ-H2AX. In single gene deletion yeast strains, the RIND-EDSB levels are altered; the number of RIND-EDSBs is higher in strains with deletions of histone deacetylase, endonucleases, topoisomerase, or DNA repair regulators, but lower in strains with deletions of the high-mobility group box proteins or Sir2. In summary, RIND-EDSBs are different from pathologic DSBs in terms of their causes and consequences. In this study, we identified the nucleotide sequences surrounding RIND-EDSBs and investigated the features of these sequences as well as their break locations. |
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